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Dobretsov G.E. Syrejschikova T.I. Smolina N.V. Uzbekov M.G.
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Human Serum Albumin (HSA): Functional Structure, Synthesis and Therapeutic Uses
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A review. CAPIDAN, a special fluorescent dye, binds to drug binding sites of human serum albumin. About 90-95 per cent of its fluorescence in serum or plasma originates from albumin-bound dye. This specificity makes it possible to study the study albumin in unfractionated serum saving intact albumin structure. Due to these properties, CAPIDAN has shown that a variety of diseases is accompanied by some changes of the drug-binding sites. This is a new source of information useful for clinics. That sensitivity to site abnormalities is raised by using time-resolved measurements of fluorescence decay and three new methods of the data analysis: (1) estimation of absolute values of concentration of fluorescent species in heterogeneous biological objects; (2) measurement of dye rotational mobility in heterogeneous albumin binding sites; and (3) method of chloride/nitrate substitution to detect interaction of a drug negative charge with arginine positive charges in the albumin binding sites.
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