Vlasova I.I. Mikhalchik E.V. Barinov N.A. Kostevich V.A., Smolina N.V., Klinov D.V., Sokolov A.V.

Nanomedicine: Nanotechnology, Biology and Medicine

2016

Vol.12, N 6

1615–1625

English

Proteins adsorbed on a surface may affect the interaction of this surface with cells. Here, we studied the binding of human serum albumin (HSA), fibrinogen (FBG) and immunoglobulin G (IgG) to PEGylated single-walled carbon nanotubes (PEG-SWCNTs) and evaluated the impact of PEG-SWCNT treated by these proteins on neutrophils in whole blood samples. Measurements of adsorption parameters revealed tight binding of proteins to PEG-SWCNTs. AFM was employed to directly observe protein binding to sidewalls of PEG-SWCNTs. Fluorescein-labeled IgG was used to ascertain the stability of PEG-SWCNT-IgG complexes in plasma. In blood samples, all plasma proteins mitigated damage of neutrophils observed just after blood exposure to PEG-SWCNTs, while only treatment of PEG-SWCNTs with IgG resulted in dose- and time-dependent enhancement of CNT-induced neutrophil activation and in potentiation of oxidative stress.

Смолина Наталья Викторовна 2016-06-09

PEGylated carbon nanotubes, Albumin, Immunoglobulin G, Fibrinogen, Neutrophil activation

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