Gryzunov Yu Koplik E. Smolina N. Kopaeva L., Dobretsov G, Sudakov K.

The International Journal on the Biology of Stress

2006

Vol.9, N 1

59-60

English

In this study, the hypothesis was tested that behaviour of rats under the open field test condition and effects of subsequent acute stress relate to conformational properties of the main plasma carrier protein, albumin. To evaluate albumin properties, fluorescence intensity of a molecular probe CAPIDAN (N-carboxyphenylimide of dimethylaminonaphthalic acid) at N (at pH 7.4) and F (at pH 4.2) albumin conformations was measured and the N–F signal ratio was calculated. The data obtained showed that CAPIDAN fluoresces selectively from albumin in rat serum and its fluorescence is sensitive to binding of fatty acids and some other ligands to albumin. Behaviour of 78 Wistar male rats was chara

Смолина Наталья Викторовна 2012-04-09

acute stress, albumin conformation, binding sites, fatty acids, fluorescent probe

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